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The presence of the parA gene transcript was inversely correlated with the maturity of nodule the transcript was switched off in fully mature bacteroids. Our data indicate that the bacA gene expression is parA dependent in symbiosis. Since the bacA gene has been considered an indicator for bacteroid formation, we applied the expression pattern of bacA as a nodule maturity index in this study. Moreover, the Δ parA cells within the nodule-like structures exhibited multiple developmental-stage phenotypes. Most showed signs of bacteroid early senescence. Upon inoculation of the Δ parA mutant onto the stem of Sesbania rostrata, three types of immature nodule-like structures with impaired nitrogen-fixing activity were generated. The Δ parA mutant showed a pleiomorphic cell shape phenotype and was polyploid, with differences in nucleoid sizes due to dramatic defects in chromosome partitioning. 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